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Recombinant CHO-K1 cells stably overexpress human low affinity immunoglobulin gamma Fc region receptor III-A (Fc RIIIa/CD16A 158V) and Fc epsilon receptor Ig (FcER1G) on the surface The surface expression of CD16A 158V is validated by FACS analysis
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High-quality, high-viability (> 90%) CD3 Pan T cells purified from rhesus monkey (or macaque) PBMCs by magnetic selection. Highly enriched (typically > 90%) post-selection purity achieved via magnetic isolation. Used for a wide variety of safety assessment and functional assays. All orders come with an iQ Certificate of Analysis.The following donor requests can be accommodated if inventory is available: - Single or multiple donors for a particular product (SKU). Indicate number of vials from each individual donor - Donor-matched PBMCs
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The Nucleofector 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector Kit is the optimal kit for efficient transfection of primary human umbilical vein endothelial cells (HUVECs).The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cellsLow-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human Umbilical Vein Endothelial Cells0.5 mL Supplement 330 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
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